Limitations in 3D reconstruction of X-rays tomography

To reconstruct the 3D information of X-rays tomography (tomoX). many authors [1, 2, 3, 4] uses algorthims implemented for electron microscopy ([5, 6]) To analyze the performance of applying EM reconstruction algorithms to tomoX microscopy we have developed an X-ray microscope simulator. This simulator combines a image formation model based on the volume information, with the characteristics of zone plates Fresnel lenses to generate the specific Point Spread Functions (PSF)[7] for each plane of the volume. In the best focused plane, we obtain a resolution of 1.22 times the width of the outer ring of the zone plate, becoming for the rest of planes a defocus effect. The differences between EM tomography and tomoX are not only the blurr effect of the optical system (Figure 1), but the influence of the depth of field along the field.